--- title: "TB-500 Peptide Supplier | China GMP Manufacturer & Wholesale – AMP Peptide" id: "830" type: "page" slug: "tb-500-peptide-supplier" published_at: "2026-07-15T02:00:00+00:00" modified_at: "2026-07-16T03:31:31+00:00" url: "https://amppeptide.com/tb-500-peptide-supplier/" markdown_url: "https://amppeptide.com/tb-500-peptide-supplier.md" excerpt: "TB-500 (Thymosin Beta-4) research peptide from GMP manufacturer. CAS 77591-33-4, ≥98% purity. Used in angiogenesis and tissue repair research. Bulk supply for labs worldwide." --- **TB-500 (Thymosin Beta-4)** | Specification | Detail | | --- | --- | | CAS No | 77591-33-4 | | Molecular Formula | Not applicable (43-amino-acid polypeptide) | | Molecular Weight | 4963.5 g/mol | | Purity | ≥98% (HPLC) | | Appearance | White lyophilized powder | | Manufacturing Standards | GMP-compliant facilities | | Pricing | TB5: $79 · TB10: $146 ( (10 vials per box) | | MOQ | 1 box (10 vials); bulk orders welcome | | Wholesale | Available for institutional and B2B clients | | Research Use Only | For laboratory and scientific research purposes only. Not for human use. | AMP Peptide supplies high-purity TB-500 (Thymosin Beta-4) as a lyophilized peptide designed for laboratory research applications. Manufactured in GMP-compliant facilities and rigorously tested via HPLC and mass spectrometry, our TB-500 meets the quality standards demanded by academic institutions, pharmaceutical R&D departments, and biotechnology laboratories worldwide. For pricing, bulk orders, or technical inquiries, [contact us](https://amppeptide.com/contact/) . [Edit form](https://amppeptide.com/wp-admin/admin.php?page=forminator-cform-wizard&id=204) ## Short Product Overview TB-500, also known as Thymosin Beta-4, is a 43-amino-acid synthetic polypeptide that represents one of the most extensively studied actin-binding peptides in regenerative research. Naturally occurring in virtually all human cells, Thymosin Beta-4 plays a fundamental role in cytoskeletal organization, cell migration, and tissue remodeling processes at the molecular level. Classified under tissue repair and regenerative research peptides, TB-500 has been investigated in numerous preclinical studies exploring its interactions with the actin cytoskeleton, its role in promoting endothelial cell migration, and its effects on extracellular matrix dynamics. ## What Is TB-500 Peptide ### Discovery and Research Background Thymosin Beta-4 was first isolated from calf thymus in 1981 by researchers investigating thymic hormone fractions. The peptide was subsequently identified as a ubiquitous protein present in nearly all mammalian cell types, with particularly high concentrations in platelets, wound fluid, and certain tissues. Its designation as TB-500 in research contexts typically refers to a stabilized synthetic version optimized for laboratory use. The complete 43-amino-acid sequence was characterized in the mid-1980s, revealing a highly conserved structure across mammalian species, suggesting fundamental biological importance. ### Amino Acid Sequence **Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser** ### Peptide Classification Actin-binding peptides / Thymosin family peptides / Tissue repair research compounds / Research-grade polypeptides ### Laboratory Applications TB-500 is widely utilized in preclinical research settings for: - In vitro cell culture studies examining cellular behavior and signaling - In vivo animal models investigating tissue-level responses - Molecular biology assays exploring signaling pathways - Biochemical characterization studies - Comparative peptide analysis research The peptide is supplied as a lyophilized powder for reconstitution in laboratory-grade solvents prior to experimental use. ## Molecular Characteristics | Parameter | Specification | | --- | --- | | Molecular Formula | Not applicable (43-amino-acid polypeptide) | | Molecular Weight | 4963.5 g/mol | | CAS Number | 77591-33-4 | | Purity | ≥98% (HPLC) | | Appearance | White lyophilized powder | | Solubility | Soluble in water, PBS, saline | | Storage Conditions | -20°C (long-term); 2-8°C (short-term, desiccated) | | Stability | Stable for 24+ months lyophilized at -20°C; reconstituted solutions stable for 7-14 days at 2-8°C | ## Research Overview TB-500 has been investigated in numerous laboratory models exploring biological mechanisms at the molecular and cellular level. ### Key Areas of Laboratory Investigation - **Cell signaling studies**: Examination of peptide-receptor interactions and downstream signaling cascades - **Cellular behavior assays**: Migration, proliferation, and differentiation studies in controlled conditions - **Biochemical pathway analysis**: Molecular mechanism investigations using purified systems - **Tissue-level research**: Ex vivo and in vivo model systems for translational research ### Receptor and Signaling Pathway Research Current understanding of TB-500’s molecular mechanism involves interactions with specific signaling pathways relevant to its peptide class. Researchers have employed various biochemical and cell-based assays to elucidate these interactions in controlled laboratory settings. ### Biological Models Studied - Rodent research models - Cell culture systems (primary and immortalized lines) - Ex vivo tissue preparations - Biochemical reconstitution systems ## How Does TB-500 Work TB-500’s primary mechanism of action revolves around its interaction with the actin cytoskeleton, the structural framework of cells. ### Actin Binding and Sequestration TB-500 functions as a G-actin (globular actin) sequestering peptide. By binding to monomeric actin, it maintains a pool of available actin subunits that can be rapidly polymerized into F-actin (filamentous actin) filaments when required for cellular processes such as migration, division, and shape changes. This actin-binding activity is central to its effects on cell motility. ### Cell Migration and Chemotaxis Thymosin Beta-4 has been shown to upregulate the expression of cell surface receptors involved in chemotaxis and migration. Studies demonstrate that TB-500 treatment increases the expression of the CD44 receptor and various integrins, facilitating cell attachment, spreading, and directed migration in laboratory models. ### Angiogenic Signaling TB-500 has been investigated for its ability to promote angiogenesis through upregulation of vascular endothelial growth factor (VEGF) and subsequent activation of the VEGF receptor signaling cascade. This effect has been observed in multiple experimental systems, including endothelial cell tube formation assays and in vivo angiogenesis models. ### Extracellular Matrix Modulation Research suggests TB-500 influences matrix metalloproteinase (MMP) expression and activity, particularly MMP-2 and MMP-9, which are involved in extracellular matrix remodeling. This modulation affects cell migration through tissue environments and contributes to the structural reorganization observed in tissue repair studies. ### Molecular Pathway Summary | Pathway | Observed Effect | Research Model | | --- | --- | --- | | Actin sequestration | G-actin binding, polymerization regulation | Biochemical assays | | CD44/integrin expression | Enhanced cell migration | Cell culture | | VEGF/VEGFR signaling | Angiogenic stimulation | Endothelial assays | | MMP-2/MMP-9 modulation | ECM remodeling | Tissue models | | PI3K/Akt pathway | Cell survival signaling | Proliferation studies | ## Manufacturing Process AMP Peptide produces TB-500 through a controlled manufacturing process designed to ensure batch-to-batch consistency and high purity. ### Step 1: Solid-Phase Peptide Synthesis (SPPS) TB-500 is synthesized using Fmoc-based solid-phase peptide synthesis chemistry. The amino acid sequence is assembled sequentially on a resin support, with each amino acid addition verified through coupling efficiency monitoring. ### Step 2: Cleavage and Deprotection Following sequence completion, the peptide is cleaved from the resin and side-chain protecting groups are removed. The crude peptide is then precipitated and washed to remove residual reagents. ### Step 3: Preparative HPLC Purification The crude peptide undergoes purification via preparative reverse-phase high-performance liquid chromatography (RP-HPLC), achieving the target purity specification. ### Step 4: Mass Spectrometry Confirmation Purified peptide is analyzed by electrospray ionization mass spectrometry (ESI-MS) to confirm molecular identity. ### Step 5: Lyophilization The purified peptide solution is lyophilized (freeze-dried) to produce a stable lyophilized powder. ### Step 6: Quality Control Testing Each production batch undergoes comprehensive QC testing as described below. ### Step 7: Packaging Lyophilized peptide is packaged under controlled conditions in sterile, graduated vials or bulk containers. ### Why Purity Matters Peptide purity is a critical parameter in laboratory research. Impurities can produce confounding results in biological assays. AMP Peptide maintains rigorous purity standards to support data integrity. ## Product Specifications | Item | Specification | | --- | --- | | Product Name | TB-500 (Thymosin Beta-4) | | CAS Number | 77591-33-4 | | Molecular Formula | Not applicable (43-amino-acid polypeptide) | | Molecular Weight | 4963.5 g/mol | | Purity | ≥98% (HPLC) | | Appearance | White lyophilized powder | | Storage | -20°C (long-term); 2-8°C (reconstituted, short-term) | | MOQ | 1 box (10 vials); bulk orders available | | Pricing | TB5 (5 mg × 10 vials): $79 · TB10 (10 mg × 10 vials): $146 | | Package Options | Vials (10/box), bulk containers, custom packaging | | Shelf Life | 24 months (lyophilized, proper storage) | | Testing Method | HPLC, ESI-MS, Amino Acid Analysis | ## Quality Control AMP Peptide implements a comprehensive quality control protocol for every batch. ### HPLC Analysis Reverse-phase HPLC is performed on each batch to determine purity. The chromatogram is analyzed for main peak purity, with our specification threshold ensuring the target peptide constitutes the vast majority of the product. ### Mass Spectrometry (ESI-MS) Electrospray ionization mass spectrometry provides molecular weight confirmation. The measured mass is compared against the calculated monoisotopic mass. ### Certificate of Analysis (COA) Each shipment includes a COA documenting: - HPLC purity percentage - HPLC chromatogram (available upon request) - ESI-MS confirmation spectrum - Peptide content - Appearance and physical description ### Additional Testing Available - **Amino Acid Analysis (AAA)**: Quantitative composition verification - **Water Content (Karl Fischer)**: Residual moisture determination - **Endotoxin Testing**: LAL assay - **Mass Spectrometry (MS/MS)**: Sequence confirmation ### Batch Traceability All batches are assigned unique lot numbers with full manufacturing records maintained for complete traceability. ## Available Specifications & Pricing | Code | Product Name | Specification | Price (USD/Box) | | --- | --- | --- | --- | | TB5 | TB-500 | 5 mg × 10 vials | $79 | | TB10 | TB-500 | 10 mg × 10 vials | $146 | | Custom | TB-500 | Bulk / Custom spec | Contact us | ### Wholesale and Bulk Orders AMP Peptide offers competitive pricing for institutional and bulk orders. Each box contains 10 vials at the specified dosage. For orders exceeding standard specifications or requiring customized packaging, please [contact us](https://amppeptide.com/contact/) for a tailored quotation. ### OEM and Private Label For qualified B2B partners, we offer OEM and private label services, including custom vial labeling, packaging design, and dedicated batch production. ## Applications in Research ### Molecular Research TB-500 is employed in molecular biology investigations examining cell signaling pathways, gene expression regulation, and protein interactions. Researchers utilize the peptide in Western blotting, ELISA, and PCR-based assays to study downstream effects of peptide treatment. ### Cell Studies In cell culture models, this peptide has been studied for its effects on cellular behavior, signaling activation, and molecular responses under controlled experimental conditions. ### Laboratory Investigations Key experimental areas include signaling pathway analysis, cellular response characterization, receptor binding studies, and biochemical mechanism investigations. ## Why Choose AMP Peptide ### GMP-Compliant Manufacturing Our production facilities operate under Good Manufacturing Practice guidelines, ensuring consistent quality across all batches. ### Rigorous Quality Testing Every batch undergoes HPLC purity analysis, mass spectrometry identity confirmation, and peptide content determination. Third-party testing available upon request. ### Global Export Experience AMP Peptide ships to research institutions, biotechnology companies, and pharmaceutical laboratories worldwide. ### Custom Manufacturing Capabilities Custom synthesis services for modified sequences, specialized purity grades, or specific requirements. ### Wholesale Supply Capacity Production capacity supports institutional and wholesale orders with consistent pricing and reliable lead times. ### Complete Documentation Certificates of Analysis included with every order. HPLC chromatograms and MS spectra available upon request. ### Technical Support Our scientific team is available for technical questions regarding handling, reconstitution, storage, and experimental applications. ## Packaging Information | Parameter | Detail | | --- | --- | | Vial Size | 2 mL, 5 mL, 10 mL (graduated, sterile) | | Vial Material | Type I borosilicate glass | | Closure | Rubber stopper with aluminum crimp seal | | Labels | Product name, quantity, purity, lot number, storage conditions | | Cold Chain Shipping | Available for temperature-sensitive orders | | Export Packaging | Triple-layer packaging compliant with international shipping regulations | | Bulk Packaging | HDPE containers with desiccant for gram-scale orders | | Documentation | COA, MSDS, packing list, commercial invoice | ## Storage Information ### Lyophilized Peptide Storage Store at **-20°C** for long-term preservation. Under these conditions, the peptide remains stable for **24 months or longer**. ### Short-Term Storage Storage at **2-8°C** in a desiccated environment is acceptable for up to 30 days. Keep vial tightly sealed and protected from moisture. ### Reconstituted Peptide Storage Store reconstituted solutions at **2-8°C** and use within **7-14 days**. Aliquot and freeze at -20°C for extended use. Avoid repeated freeze-thaw cycles. ### Stability Considerations - **Temperature**: Maintain cold storage - **Moisture**: Minimize ambient humidity exposure - **pH**: Optimal stability at pH 4-7 - **Oxidation**: Protect from prolonged atmospheric oxygen exposure ## Related Products Researchers interested in this product frequently investigate the following related products: - [BPC-157 Peptide Supplier](https://amppeptide.com/bpc-157-peptide-supplier/) - [Semaglutide Peptide Manufacturer](https://amppeptide.com/semaglutide-peptide-manufacturer/) - [GHK-Cu Peptide Supplier](https://amppeptide.com/ghk-cu-peptide-supplier/) - [Tirzepatide Peptide Supplier](https://amppeptide.com/tirzepatide-peptide-supplier/) - [Retatrutide Peptide Supplier](https://amppeptide.com/retatrutide-peptide-supplier/) - [NAD+ Peptide Manufacturer](https://amppeptide.com/nad-peptide-manufacturer/) - [MOTS-c Peptide Supplier](https://amppeptide.com/mots-c-peptide-supplier/) - [Epithalon Peptide Supplier](https://amppeptide.com/epithalon-peptide-supplier/) - [DSIP Peptide Supplier](https://amppeptide.com/dsip-peptide-supplier/) - [Thymosin Alpha-1 Peptide Supplier](https://amppeptide.com/thymosin-alpha-1-peptide-supplier/) - [Ipamorelin Peptide Supplier](https://amppeptide.com/ipamorelin-peptide-supplier/) - [GHRP-2 Peptide Supplier](https://amppeptide.com/ghrp-2-peptide-supplier/) - [GHRP-6 Peptide Supplier](https://amppeptide.com/ghrp-6-peptide-supplier/) - [Kisspeptin-10 Peptide Supplier](https://amppeptide.com/kisspeptin-10-peptide-supplier/) - [VIP Peptide Supplier](https://amppeptide.com/vip-peptide-supplier/) - [IGF-1 LR3 Peptide Supplier](https://amppeptide.com/igf-1-lr3-peptide-supplier/) - [Oxytocin Peptide Supplier](https://amppeptide.com/oxytocin-peptide-supplier/) - [PT-141 Peptide Supplier](https://amppeptide.com/pt-141-peptide-supplier/) - [Ara-290 Peptide Supplier](https://amppeptide.com/ara-290-peptide-supplier/) - [SS-31 Peptide Supplier](https://amppeptide.com/ss-31-peptide-supplier/) - [Melanotan I Peptide Supplier](https://amppeptide.com/melanotan-i-peptide-supplier/) - [Melanotan II Peptide Supplier](https://amppeptide.com/melanotan-ii-peptide-supplier/) - [CJC-1295 Peptide Supplier](https://amppeptide.com/cjc-1295-peptide-supplier/) - [Tesamorelin Peptide Supplier](https://amppeptide.com/tesamorelin-peptide-supplier/) - [AOD-9604 Peptide Supplier](https://amppeptide.com/aod-9604-peptide-supplier/) - [Snap-8 Peptide Supplier](https://amppeptide.com/snap-8-peptide-supplier/) - [Semax Peptide Supplier](https://amppeptide.com/semax-peptide-supplier/) - [Selank Peptide Supplier](https://amppeptide.com/selank-peptide-supplier/) - [AHK-Cu Peptide Supplier](https://amppeptide.com/ahk-cu-peptide-supplier/) - [5-Amino-1MQ Research Compound](https://amppeptide.com/5-amino-1mq-research-compound/) - [Explore Our Complete Peptide Product Catalog](https://amppeptide.com/amp-peptide-product-list/) ( ## FAQ Section ) For a complete overview of all available research peptides and pricing, visit our [complete peptide product catalog](https://amppeptide.com/amp-peptide-product-list/) . ### What is TB-500 peptide? TB-500 is a synthetic peptide supplied for laboratory research purposes. It is designed for use in controlled research settings investigating cellular signaling, molecular mechanisms, and biological pathway analysis. ### Is TB-500 suitable for laboratory research? Yes, this product is manufactured specifically for laboratory and scientific research purposes. It is not intended for human consumption or medical use. ### How should this peptide be stored? Lyophilized peptide should be stored at -20°C for long-term preservation. For short-term use, storage at 2-8°C in a desiccated environment is acceptable. Avoid repeated freeze-thaw cycles after reconstitution. ### What purity specifications are available? Our standard purity is ≥98% (HPLC) as determined by HPLC analysis. Custom specifications are available upon request. ### What testing methods are used? Each batch is tested using reverse-phase HPLC for purity determination and ESI-MS for molecular weight identity confirmation. ### Is wholesale available? Yes, AMP Peptide offers wholesale pricing for bulk orders. Volume-based pricing is available for institutional and B2B clients. ### Can custom packaging be provided? Yes, OEM and private label services are available for qualified B2B partners, including custom packaging and labeling. ### Does AMP Peptide provide COA documents? Yes, every order includes a Certificate of Analysis with full analytical documentation. Additional data is available upon request. ### What industries use this peptide? Academic research institutions, pharmaceutical R&D laboratories, biotechnology companies, and contract research organizations (CROs). ### What shipping methods are available? Standard and express shipping are available for domestic and international orders. Cold chain shipping is available for temperature-sensitive protocols. ### What is the shelf life? 24 months or longer when stored properly at -20°C in lyophilized form. ### Is this product legal for research purposes? This peptide is supplied exclusively for laboratory research purposes. Researchers should verify compliance with local regulations governing research peptide procurement and use in their jurisdiction. ## Research Use Disclaimer **This product is supplied exclusively for laboratory and scientific research purposes.** It is not intended for human consumption, medical use, diagnosis, treatment, or disease prevention. This peptide has not been evaluated by the FDA or any other regulatory body for safety or efficacy in humans. The product is intended for use by qualified researchers in controlled laboratory settings only. AMP Peptide makes no claims regarding therapeutic or medical benefits. All products are manufactured for research purposes and sold with the understanding that the purchaser will use them in compliance with all applicable laws and regulations.